The HRMSF currently has four mass spectrometers:

Thermo Q-Exactive (Orbitrap)

A Thermo Q-Exactive was installed in January 2020 and purchased with funds from the NSF Major Research Instrumentation and the Chemical Instrumentation Programs (1919422). The Q-Exactive is an Orbitrap mass spectrometer capable of very high resolution measurement of mass-to-charge ratios and tandem mass spectrometry (MS/MS), that will support the identification and quantification of novel products and processes. Samples can be directly infused to the instrument or pre-separated by ultra performance liquid chromatography (UPLC). An on-line diode array detector (DAD) enables compound detection by ultraviolet and visible (UV-Vis) light prior to MS detection.

Waters Xevo TQ-S cronos

In February 2020, we installed a Waters Xevo TQ-S cronos which is a triple quadrupole mass spectrometer interfaced with an Acquity H-Class UPLC.  This LC-MS is mainly used for its multiple reaction monitoring (MRM) capabilities trace level quantitative analysis.  The TQ-S also has other MS/MS scanning capabilities that allow us to run precursor (parent) ion scans, product (daughter) ion scans, and constant neutral loss scans, all of which can be used for structure elucidation

Waters GCT Premier

A Waters GCT Premier was installed in early 2011 and purchased with funds from the NSF CRIF multi-user instrument grant (0946779).  The GCT is a gas chromatograph combined with a time-of-flight mass spectrometer.  This instrument can be used to perform GC-MS with either electron ionization (EI) or chemical ionization (CI).  If samples are not amendable to GC-MS, we have capabilities to use either a solids probe or desorption chemical ionization probe (DCI) to introduce a sample into the mass spectrometer. We can use this instrument to perform high resolution or accurate mass measurement experiments (HR-EI and HR GC-MS)

Waters Q-Tof Premier

A Waters Q-Tof Premier is a high resolution hybrid quadrupole time-of-flight mass spectrometer.  The Q-Tof Premier has both electrospray ionization (ESI) and atomospheric pressure chemical ionization (APCI) capabilities.  It is has LC-MS capabilities when interfaced with our Waters Acquity ultra-high pressure liquid chromatography system (UPLC).  The Acquity UPLC includes a tunable wavelength detector (TUV) and autosampler that holds two plates each with 48 vial positions or two 96-well plates.  The Q-TOF Premier also is capable of performing tandem mass spectrometry (MS/MS) experiments.  This mass spectrometer was purchased with funds from a NIH Shared Instrumentation Grant (S10 RR023384-01) and installed in 2008.  The facility has a second Q-Tof Premier that is used for direct infusion analysis. 



After a brief training session, interested University researchers have the opportunity to analyze their own samples on the any of the mass spectrometers.  Slightly more involved training is necessary when learning to perform LC-MS, MS-MS, and HR-MS experiments. Numerous graduate students, staff, and postdocs from Chemistry, Pharmacy, Human Toxicology, and other departments and colleges across campus are trained to operate the mass spectrometers each year.