The HRMSF currently has four mass spectrometers:
Waters GCT Premier
A Waters GCT Premier is our newest mass spectrometer. It was installed in early 2011 and purchased with funds from the NSF CRIF multi-user instrument grant (0946779). The GCT is a gas chromatograph combined with a time-of-flight mass spectrometer. This instrument can be used to perform GC-MS with either electron ionization (EI) or chemical ionization (CI). If samples are not amendable to GC-MS, we have capabilities to use either a solids probe or desorption chemical ionization probe (DCI) to introduce a sample into the mass spectrometer. We can use this instrument to perform high resolution or accurate mass measurement experiments (HR-EI and HR GC-MS)
Waters Q-Tof Premier
A Waters Q-Tof Premier is a high resolution hybrid quadrupole time-of-flight mass spectrometer. The Q-Tof Premier has both electrospray ionization (ESI) and atomospheric pressure chemical ionization (APCI) capabilities. It is has LC-MS capabilities when interfaced with our Waters Acquity ultra-high pressure liquid chromatography system (UPLC). The Acquity UPLC includes a tunable wavelength detector (TUV) and autosampler that holds two plates each with 48 vial positions or two 96-well plates. The Q-TOF Premier also is capable of performing tandem mass spectrometry (MS/MS) experiments. This mass spectrometer was purchased with funds from a NIH Shared Instrumentation Grant (S10 RR023384-01) and installed in 2008. The facility has a second Q-Tof Premier that is used for direct infusion analysis.
Waters Acquity TQD
In March 2012, we installed a Waters Acquity TQD which is a triple quadrupole mass spectrometer with a Acquity H Class UPLC. This instrument has ESI capabilities. The TQD can be used to directly infuse samples for nominal mass determination. The TQD also has MS/MS capabilities that allow us to run precursor (parent) ion scans, product (daughter) ion scans, and constant neutral loss scans, all of which can be used for structure elucidation. Multiple reactions monitoring (MRM) can also be performed on the TQD for quantitative analysis.
After a brief training session, interested University researchers have the opportunity to analyze their own samples on the any of the mass spectrometers. Slightly more involved training is necessary when learning to perform LC-MS, MS-MS, and HR-MS experiments. Numerous graduate students, staff, and postdocs from Chemistry, Pharmacy, Human Toxicology, and other departments and colleges across campus are trained to operate the mass spectrometers each year.