The HRMSF currently has four mass spectrometers:

Thermo Q-Exactive (Orbitrap)

A Thermo Q-Exactive was installed in January 2020 and purchased with funds from the NSF Major Research Instrumentation and the Chemical Instrumentation Programs (1919422). The Q-Exactive is an Orbitrap mass spectrometer capable of very high resolution measurement of mass-to-charge ratios and tandem mass spectrometry (MS/MS), that will support the identification and quantification of novel products and processes. Samples can be directly infused to the instrument or pre-separated by ultra performance liquid chromatography (UPLC). An on-line diode array detector (DAD) enables compound detection by ultraviolet and visible (UV-Vis) light prior to MS detection.


Waters Xevo TQ-S cronos

In February 2020, we installed a Waters Xevo TQ-S cronos which is a triple quadrupole mass spectrometer interfaced with an Acquity H-Class UPLC.  This LC-MS is mainly used for its multiple reaction monitoring (MRM) capabilities trace level quantitative analysis.  The TQ-S also has other MS/MS scanning capabilities that allow us to run precursor (parent) ion scans, product (daughter) ion scans, and constant neutral loss scans, all of which can be used for structure elucidation


Waters GCT Premier

A Waters GCT Premier was purchased with funds from the NSF CRIF multi-user instrument grant (0946779).  The GCT is a gas chromatograph combined with a time-of-flight mass spectrometer.  This instrument can be used to perform GC-MS with either electron ionization (EI) or chemical ionization (CI).  If samples are not amendable to GC-MS, we have capabilities to use either a solids probe or desorption chemical ionization probe (DCI) to introduce a sample into the mass spectrometer. We can use this instrument to perform high resolution or accurate mass measurement experiments (HR-EI and HR GC-MS)


Waters SQD

A Waters SQD is a single quadrupole mass spectrometer.   It has LC-MS capabilities when interfaced with our Waters Acquity ultra-high pressure liquid chromatography system (UPLC).  The Acquity UPLC includes a tunable wavelength detector (TUV) and autosampler that holds two plates each with 48 vial positions or two 96-well plates.   This LC-MS is available for walk-on users who need to confirm molecular weight on isolated products or check the course of an experimental synthesis.  The system can also be used for LC method development (gradient and column determination) prior to lengthy sample analysis runs on one of the other Facility instruments



After a brief training session, interested University researchers have the opportunity to analyze their own samples on the any of the mass spectrometers.  Slightly more involved training is necessary when learning to perform LC-MS, MS-MS, and HR-MS experiments. Numerous graduate students, staff, and postdocs from Chemistry, Pharmacy, Human Toxicology, and other departments and colleges across campus are trained to operate the mass spectrometers each year.